MAJOR LINKS FOR CD53  

• NCBI LocusLink Record: 963
• Mendelian Inheritance in Man (OMIM): 151525
• SwissProt annotated protein record: P19397

CELLULAR FUNCTION OF CD53  

• Signal transduction
• CD53 cross-linking promotes activation of human B cells and rat macrophages

DISEASE RELEVANCE OF CD53 AND FUNCTION OF CD53 IN INTACT ANIMAL   - No information

• Families in which CD53 is a member
  • CD53-->TM4SF

MOLECULAR STRUCTURE OF CD53  

• 219 amino acids
• CD53 is a type III membrane protein, with four putative transmembrane domains, delimiting two unequal extracellular loops
• The short ends are located in the cytoplasm. A signature of the tetraspan superfamily is present between transmembrane domains II and III: G-x(3)-[LIVMF]-x(2)-[GSA]-[LIVMF](2)-G-C-x-[GA]-[STA]-x(2)-[EG]-x(2)-[CW]-[LIVM](2)

MOLECULAR MASS OF CD53  

POST-TRANSCRIPTIONAL MODIFICATION OF CD53   - No information

POST-TRANSLATIONAL MODIFICATION OF CD53  

• Two N-glycosylation sites present in the large extracellular region
• The Mr is dropped to about 25 kDa upon treatment with endoglycosidase F

SUBSTRATES FOR CD53   - No information

ENZYMES WHICH MODIFY CD53   - No information

LIGANDS FOR CD53 AND MOLECULES ASSOCIATED WITH CD53  

• Tetraspans, integrins and MHC class II molecules may be in a single large complex
• In rat, CD53 is associated with CD2 and a tyrosine phosphatase activity

• Leukocytes
• Not present on platelets, red blood cells and non-hematopoietic cells

SELECTION OF OTHER CD53-SPECIFIC REFERENCE MAB  

REVIEWS

1. Wright MD,Tomlinson MG The ins and outs of the transmembrane 4 superfamily. Immunol Today 1994 15:588 PubMed

PRIMARY CITATIONS

2. Amiot M Identification and analysis of cDNA clones encoding CD53. A pan- leukocyte antigen related to membrane transport proteins. J Immunol 1990 145:4322 PubMed

3. Angelisova P,Hilgert I,Horejsi V Association of four antigens of the tetraspans family (CD37, CD53, TAPA- 1, and R2/C33) with MHC class II glycoproteins. Immunogenetics 1994 39:249 PubMed

4. Angelisova P,Vlcek C,Stefanova I,Lipoldova M,Horejsi V The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX-44 antigens. Immunogenetics 1990 32:281 PubMed

5. Bell GM,Seaman WE,Niemi EC,Imboden JB The OX-44 molecule couples to signaling pathways and is associated with CD2 on rat T lymphocytes and a natural killer cell line. J Exp Med 1992 175:527 PubMed

6. Bosca L,Lazo PA Induction of nitric oxide release by MRC OX-44 (anti-CD53) through a protein kinase C-dependent pathway in rat macrophages. J Exp Med 1994 179:1119 PubMed

7. Carmo AM,Wright MD Association of the transmembrane 4 superfamily molecule CD53 with a tyrosine phosphatase activity. Eur J Immunol 1995 25:2090 PubMed

8. Mannion BA,Berditchevski F,Kraeft SK,Chen LB,Hemler ME Transmembrane-4 superfamily proteins CD81 (TAPA-1), CD82, CD63, and CD53 specifically associated with integrin alpha 4 beta 1 (CD49d/CD29). J Immunol 1996 157:2039 PubMed

9. Mitnacht R,Tacke M,Hunig T Expression of cell interaction molecules by immature rat thymocytes during passage through the CD4+8+ compartment: developmental regulation and induction by T cell receptor engagement of CD2, CD5, CD28, CD11a, CD44 and CD53. Eur J Immunol 1995 25:328 PubMed

10. Olweus J,Lund-Johansen F,Horejsi V CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells. J Immunol 1993 151:707 PubMed

11. Rasmussen AM,Blomhoff HK,Stokke T,Horejsi V,Smeland EB Cross-linking of CD53 promotes activation of resting human B lymphocytes. J Immunol 1994 153:4997 PubMed

WWW RESOURCES

Portions copyright by Garland Press and by the International Workshops on Human Leukocyte Differentiation Antigens; used with permission

Modified 10/14/99   mpr@mail.nih.gov