• Fcalpha-receptor R, Fcalpha-R
• IgA Fc receptor
• IgA receptor

MAJOR LINKS FOR CD89  

• NCBI LocusLink Record: 2204
• Mendelian Inheritance in Man (OMIM): 147045
• SwissProt annotated protein record: P24071

• Binds both monomeric and polymeric forms of either IgA1 or IgA2 at the boundary between the Calpha2 and Calpha3 domains

CELLULAR FUNCTION OF CD89  

• Induces phagocytosis
• Induces degranulation
• Induces respiratory burst
• Induces killing of microorganisms

DISEASE RELEVANCE OF CD89 AND FUNCTION OF CD89 IN INTACT ANIMAL  

• More glycosylated forms on activated eosinophils from allergic individuals and on neutrophils and monocytes from cirrhotic individuals

• Families in which CD89 is a member
  • CD89-->Fc receptor-->immunoglobulin supergene family

MOLECULAR STRUCTURE OF CD89  

• A type I transmembrane glycoprotein of 266 amino acids
• Two extracellular Ig-like (C2 type) domains
• A 19 amino acid single transmembrane segment containing a charged Arg residue
• A 41 amino acid cytoplasmic tail

MOLECULAR MASS OF CD89  

POST-TRANSCRIPTIONAL MODIFICATION OF CD89  

• Various splice variants: Transcripts lacking either the S2 mini-exon, the entire EC2 exon, or the 3' part of the EC2 exon
• Presence of mRNA instability sequences (ATTTA or TTATTTA) in the 3' untranslated region

POST-TRANSLATIONAL MODIFICATION OF CD89   - Glycosylation

SUBSTRATES FOR CD89   - N/A

ENZYMES WHICH MODIFY CD89   - N/A

LIGANDS FOR CD89 AND MOLECULES ASSOCIATED WITH CD89  

• Myeloid-lineage cells from promyelocytes to neutrophils and from promonocyte to monocytes
• Activated eosinophils
• Alveolar and splenic macrophages

• Need to define the functional form of CD89 in terms of ligand binding and signal transduction and the role of CD89 in the process of infectious diseases
• The mouse CD89 homologue has not yet been characterized either at the protein or the DNA level

SELECTION OF OTHER CD89-SPECIFIC REFERENCE MAB  

REVIEWS

1. Kerr MA The structure and function of human IgA. Biochem J 1990 271:285 PubMed

2. Shen L Receptors for IgA on phagocytic cells. Immunol Res 1992 11:273 PubMed

3. Morton HC,van Egmond M,van de Winkel JG Structure and function of human IgA Fc receptors (Fc alpha R). Crit Rev Immunol 1996 16:423 ** PubMed

PRIMARY CITATIONS

4. Shen L,Lasser R,Fanger MW My 43, a monoclonal antibody that reacts with human myeloid cells inhibits monocyte IgA binding and triggers function. J Immunol 1989 143:4117 PubMed

5. Monteiro RC,Kubagawa H,Cooper MD Cellular distribution, regulation, and biochemical nature of an Fc alpha receptor in humans. J Exp Med 1990 171:597 PubMed

6. Maliszewski CR,March CJ,Schoenborn MA,Gimpel S,Shen L Expression cloning of a human Fc receptor for IgA. J Exp Med 1990 172:1665 PubMed

7. Monteiro RC,Cooper MD,Kubagawa H Molecular heterogeneity of Fc alpha receptors detected by receptor- specific monoclonal antibodies. J Immunol 1992 148:1764 PubMed

8. Pfefferkorn LC,Yeaman GR Association of IgA-Fc receptors (Fc alpha R) with Fc epsilon RI gamma 2 subunits in U937 cells. Aggregation induces the tyrosine phosphorylation of gamma 2. J Immunol 1994 153:3228 PubMed

9. Morton HC,van den Herik-Oudijk IE,Vossebeld P,Snijders A,Verhoeven AJ,Capel PJ,van de Winkel JG Functional association between the human myeloid immunoglobulin A Fc receptor (CD89) and FcR gamma chain. Molecular basis for CD89/FcR gamma chain association. J Biol Chem 1995 270:29781 * PubMed

10. Patry C,Sibille Y,Lehuen A,Monteiro RC Identification of Fc alpha receptor (CD89) isoforms generated by alternative splicing that are differentially expressed between blood monocytes and alveolar macrophages. J Immunol 1996 156:4442 PubMed

11. Morton HC,Schiel AE,Janssen SW,van de Winkel JG Alternatively spliced forms of the human myeloid Fc alpha receptor (CD89) in neutrophils. Immunogenetics 1996 43:246 ** PubMed

12. Carayannopoulos L,Hexham JM,Capra JD Localization of the binding site for the monocyte immunoglobulin (Ig) A- Fc receptor (CD89) to the domain boundary between Calpha2 and Calpha3 in human IgA1. J Exp Med 1996 183:1579 PubMed

WWW RESOURCES

Portions copyright by Garland Press and by the International Workshops on Human Leukocyte Differentiation Antigens; used with permission

Modified 10/14/99   mpr@mail.nih.gov