[PROW BAR]
PROW and IWHLDA present the GUIDE on:
CD95
Author: Shin Yonehara
 Reviewer: Abul K. Abbas
Link to additional info in FORUM
[GUIDE BAR]
OVERVIEW
MOLECULAR FAMILIES  
  • Families in which CD95 is a member

    ALTERNATE NAMES  

  • APO-1
  • fas antigen, Fas
  • tumor necrosis factor receptor superfamily, member 6, TNFRSF6
  • apoptosis antigen 1, APT1

    MAJOR LINKS  

  • NCBI LocusLink Record: 355
  • Mendelian Inheritance in Man (OMIM): 134637
  • SwissProt annotated protein record: P25445

    • AUTHOR'S ADDITIONAL INSIGHTS  
    - No information

    SYSTEM VIEW
    DISEASE RELEVANCE / FUNCTION IN INTACT Link to additional info in FORUM  
  • lpr/lpr mutant mice have defect in Fas expression and develop lymphadenopathy and systemic autoimmune disease
  • One of receptor molecules mediating apoptosis of targets by cytotoxic T cells

    CELLULAR FUNCTION  

  • Mediation of apoptosis-inducing signals

    CELLULAR EXPRESSION  

  • Expressed, typically at high levels, on activated T and B cells
    GENE
    TRANSCRIPTION REGULATING MOLECULES  
    - No information

    TRANSCRIPT
    POST-TRANSCRIPTIONAL MODIFICATION  
  • Alternative splicing yields soluble forms of Fas
    PROTEIN
    BIOCHEMICAL ACTIVITY  
    - No information

    PROTEIN SEQUENCE LINKS  

  • SwissProt annotated protein record: P25445

    • PROTEIN SEQUENCE ANNOTATION  

  • Type I transmembrane molecule with 320 amino acids
  • 157 amino acid extracellular domain
  • Distinctive repeats of three cysteine-rich domains
  • Death domain with 214-295 amino acid (intracytoplasmic)

    PROTEIN PHYSICAL PROPERTIES  

    CELL TYPEMW UNREDUCEDMW REDUCEDComment
    Activated T cell 45, 90 >200 kDa 45 kDa  

    POST-TRANSLATIONAL MODIFICATION  

  • Two serine/threonine glycosylation sites at extracellular region
  • Phosphorylation
    MOLECULAR INTERACTIONS
    SUBSTRATES  
    MOLECULECOMMENT
    FADD/MORT1 Association of FADD through death domain-death domain interaction of Fas and FADD
    FLICE/MACH-1/caspase-8 Another domain of FADD associates with FLICE/MACH-1, a member of caspase, and then FLICE/MACH-1 is activated

    ENZYME FOR WHICH THIS IS A SUBSTRATE  
    - No information

    OTHER LIGANDS AND ASSOCIATED MOLECULES  

    MOLECULECOMMENT
    Fas ligand Fas ligand belongs to the tumor necrosis factor family
    REAGENTS
    MABS NEW  
    NAME(Workshop IDs)SOURCE or REFERENCECOMMENT
    DX2    
    B.D29    
    B.E28    
    B.G27    
    B.G30    
    B.G34    
    B.K14    
    BL25    

    MABS REFERENCE  

    NAME(Workshop IDs)SOURCE or REFERENCECOMMENT
    7C11    
    CH-11 S Yonehara et al. 1989  
    APO-1 B Trauth et al. 1989  
    REFERENCES
    REVIEWS  
    1. Krammer PH, Westendorp MO, Dhein J, Walczak H, Behrmann I, Mariani S, Matiba B, Fath M, Daniel PT and Knipping E. 1994. The role of APO-1-mediated apoptosis in the immune system. Immunol Rev 142:175-191 PubMed
    2. Nagata S and Golstein P. 1995. The Fas death factor. Science 267:1449-1456 PubMed

    PRIMARY CITATIONS  
    3. Nishimura Y, Ishii A and Yonehara S. . Expression and function of apoptosis antigen Fas on T lymphocytes in periphery and thymus. In Schlossman SF, Boumsell L, Gilks W, Harlan JM, Kishimoto T, Morimoto C, Ritz J, Shaw S, Silverstein R, Springer T, Tedder TF and Todd RF. 1995. Leukocyte Typing V. OXFORD University Press p1144
    4. Itoh N, Yonehara S, Ishii A, Yonehara M, Mizushima S, Sameshima M, Hase A, Seto Y and Nagata S. 1991. The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis. Cell 66:233-243 PubMed
    5. Rouvier E, Luciani MF and Golstein P. 1993. Fas involvement in Ca(2+)-independent T cell-mediated cytotoxicity. J Exp Med 177:195-200 PubMed
    6. Suda T, Takahashi T, Golstein P and Nagata S. 1993. Molecular cloning and expression of the Fas ligand, a novel member of the tumor necrosis factor family. Cell 75:1169-1178 PubMed
    7. Trauth BC, Klas C, Peters AM, Matzku S, Moller P, Falk W, Debatin KM and Krammer PH. 1989. Monoclonal antibody-mediated tumor regression by induction of apoptosis. Science 245:301-305 PubMed
    8. Yonehara S, Ishii A and Yonehara M. 1989. A cell-killing monoclonal antibody (anti-Fas) to a cell surface antigen co-downregulated with the receptor of tumor necrosis factor. J Exp Med 169:1747-1756 PubMed

    WWW RESOURCES  

    * indicates amended by reviewer, ** indicates added by reviewer
    Portions copyright by Garland Press and by the International Workshops on Human Leukocyte Differentiation Antigens; used with permission
    Modified 12/08/99   mpr@mail.nih.gov