• NCA-160
• biliary glycoprotein, BGP

MAJOR LINKS FOR CD66a  

• NCBI LocusLink Record: 634
• Mendelian Inheritance in Man (OMIM): 109770
• SwissProt annotated protein record: P13688

CELLULAR FUNCTION OF CD66a  

• Unclear, but capable of homophilic adhesion, heterophilic adhesion, E-selectin binding, tumor suppression, type I fimbriae binding, and transmembrane signaling
• Capable of activating neutrophils
• In addition CD66a functions as a receptor for Neisseria gonorrhea and N. meningitidis

DISEASE RELEVANCE OF CD66a AND FUNCTION OF CD66a IN INTACT ANIMAL  

• Unclear, see cellular function

• Families in which CD66a is a member
  • CD66a-->carcinoembryonic antigen-->immunoglobulin supergene family

MOLECULAR STRUCTURE OF CD66a  

• Leader 34aa, extracellular 380aa, TM 32aa, cytoplasmic 71aa
• Short cytoplasmic tail with 2 YXXL motifs
• 3 Ig C2 domains and 1 Ig-V like domain

MOLECULAR MASS OF CD66a  

POST-TRANSCRIPTIONAL MODIFICATION OF CD66a  

• 13 different splice variants have been described

POST-TRANSLATIONAL MODIFICATION OF CD66a  

• Heavily and variably N-glycosylated, (20 N-glycosylation sites), two potential phosphorylation sites in cytoplasmic domain

SUBSTRATES FOR CD66a   - No information

ENZYMES WHICH MODIFY CD66a  

LIGANDS FOR CD66a AND MOLECULES ASSOCIATED WITH CD66a Link to additional info in FORUM  

• Granulocytes and epithelial cells

• The data are consistent with the hypothesis that CD66a plays a signaling role and regulates the adhesion activity of CD11/CD18 in neutrophils. While the details of the "activation signal" transmitted by CD66 antigens are not known, the finding of tyrosine kinase activity associated with CD66a, CD66b, and CD66c suggests that these kinase activities may be involved in signal transduction via CD66 family members
• The transient changes in phosphorylation of CD66a in neutrophils following stimulation with chemotactic agents suggest that phosphorylation may be involved in regulating CD66a function as well
• Data also suggest that CD66a is strongly down regulated in malignancies and may have tumor suppressor activity

SELECTION OF OTHER CD66a-SPECIFIC REFERENCE MAB  

REVIEWS

Virji M,Watt SM,Barker S,Makepeace K,Doyonnas R The N-domain of the human CD66a adhesion molecule is a target for Opa proteins of Neisseria meningitidis and Neisseria gonorrhoeae. Mol Microbiol 1996 22:929 PubMed

PRIMARY CITATIONS

1. Svenberg T,Hammarstrom S,Zeromski J Immunofluorescence studies on the occurrence and localization of the CEA-related biliary glycoprotein I (BGP I) in normal human gastrointestinal tissues. Clin Exp Immunol 1979 36:436 PubMed

2. Audette M,Buchegger F,Schreyer M,Mach JP Monoclonal antibody against carcinoembryonic antigen (CEA) identifies two new forms of crossreacting antigens of molecular weight 90,000 and 160,000 in normal granulocytes. Mol Immunol 1987 24:1177 PubMed

3. Thompson JA,Grunert F,Zimmermann W Carcinoembryonic antigen gene family: molecular biology and clinical perspectives. J Clin Lab Anal 1991 5:344 PubMed

4. Barnett TR,Kretschmer A,Austen DA,Goebel SJ,Hart JT,Elting JJ,Kamarck ME Carcinoembryonic antigens: alternative splicing accounts for the multiple mRNAs that code for novel members of the carcinoembryonic antigen family. J Cell Biol 1989 108:267 PubMed

5. Rojas M,Fuks A,Stanners CP Biliary glycoprotein, a member of the immunoglobulin supergene family, functions in vitro as a Ca2(+)-dependent intercellular adhesion molecule. Cell Growth Differ 1990 1:527 PubMed

6. Hauck W,Nedellec P,Turbide C,Stanners CP,Barnett TR,Beauchemin N Transcriptional control of the human biliary glycoprotein gene, a CEA gene family member down-regulated in colorectal carcinomas. Eur J Biochem 1994 223:529 PubMed

7. Neumaier M,Paululat S,Chan A,Matthaes P,Wagener C Biliary glycoprotein, a potential human cell adhesion molecule, is down- regulated in colorectal carcinomas. Proc Natl Acad Sci U S A 1993 90:10744 PubMed

8. Stocks SC,Kerr MA Stimulation of neutrophil adhesion by antibodies recognizing CD15 (Le(X)) and CD15-expressing carcinoembryonic antigen-related glycoprotein NCA-160. Biochem J 1992 288:23 PubMed

9. Skubitz KM,Campbell KD,Ahmed K,Skubitz AP CD66 family members are associated with tyrosine kinase activity in human neutrophils. J Immunol 1995 155:5382 PubMed

10. Skubitz KM,Campbell KD,Skubitz AP CD66a, CD66b, CD66c, and CD66d each independently stimulate neutrophils. J Leukoc Biol 1996 60:106 PubMed

11. Kuijpers TW,Hoogerwerf M,van der Laan LJ,Nagel G,van der Schoot CE,Grunert F,Roos D CD66 nonspecific cross-reacting antigens are involved in neutrophil adherence to cytokine-activated endothelial cells. J Cell Biol 1992 118:457 PubMed

WWW RESOURCES

Portions copyright by Garland Press and by the International Workshops on Human Leukocyte Differentiation Antigens; used with permission

Modified 10/14/99   mpr@mail.nih.gov