PROW: CD27

PROW and IWHLDA present the GUIDE on:
CD27
Authors: Tetsuji Kobata; Chikao Morimoto
Reviewer: Rene A. van Lier
[GUIDE BAR]

ALTERNATE NAMES FOR CD27

S152
T14

MAJOR LINKS FOR CD27

NCBI LocusLink Record: 939
Mendelian Inheritance in Man (OMIM): 186711
SwissProt annotated protein record: P26842

FUNCTION BIOCHEMICAL ACTIVITY OF CD27

Tyrosine-phosphorylation of ZAP-70 following CD27 ligation of T cells has been reported (Kobata et al. 1994), but not confirmed independently*

CELLULAR FUNCTION OF CD27

CD27 mediates a co-stimulatory signal for T and B cell activation and is involved in murine T cell development

DISEASE RELEVANCE OF CD27 AND FUNCTION OF CD27 IN INTACT ANIMAL

Levels of soluble CD27 in biological fluids increase in patients suffering from a variety of immunopathological diseases as a marker for T cell activation in vivo

STRUCTURE MOLECULAR FAMILY FOR CD27

Families in which CD27 is a member
- CD27-->TNF-R

MOLECULAR STRUCTURE OF CD27

Type I transmembrane molecule (240 amino acids)
171 amino acid extracellular domain with 16 clustered cysteines and a homologous domain to TNF receptor
21 amino acid hydrophobic transmembrane domain and 48 amino acid intracytoplasmic domain with a site for serine phosphorylation

MOLECULAR MASS OF CD27

CELL TYPE	MW UNREDUCED	MW REDUCED	Comment
T lymphocytes	110-120 kDa	55 kDa

POST-TRANSCRIPTIONAL MODIFICATION OF CD27

CD27 cDNA does not contain downstream poly(A) tract, causing the size difference between the cDNA (1.2 kb) and the mRNA (1.3 kb)

POST-TRANSLATIONAL MODIFICATION OF CD27

One N-linked and three O-linked glycosylation sites in the extracellular domain
Soluble CD27 is produced by lymphocytes upon activation, probably due to proteolytic cleavage
CD27 undergoes serine-phosphorylation in the intracytoplasmic domain

MOLECULAR INTERACTIONS PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD27 - No information

SUBSTRATES FOR CD27 - No information

ENZYMES WHICH MODIFY CD27 - No information

LIGANDS FOR CD27 AND MOLECULES ASSOCIATED WITH CD27

MOLECULE	COMMENT
CD70	Type II transmembrane molecule belonging to the TNF family
SIVA	Apoptosis-inducing protein reported (Prasad et al. 1997) but not confirmed independently**
TRAF5	Involved in NF-kappaB and SAPK/JNK activation by CD27 (Akiba H et al. 1998)**
TRAF2	Involved in NF-kappaB and SAPK/JNK activation by CD27 (Akiba H et al. 1998)**

EXPRESSION MAIN CELLULAR EXPRESSION OF CD27

Expressed on majority of peripheral blood T cells
Expressed preferentially on CD45RA+ T cells after activation and medullary thymocytes
Expressed on memory-type B cells. B-cells acquire CD27 expression in the germinal center (GC) and keep on expressing the molecule until the plasma cell stage*
Expressed on NK cells

AUTHOR'S ADDITIONAL INSIGHTS ON CD27

CD27/CD70 interactions regulate B cell proliferation and differentiation by T cells, in addition to T cell activation

REAGENTS CD27-SPECIFIC MABS NEWLY ASSIGNED AT SIXTH INTERNATIONAL WORKSHOP

NAME(Workshop IDs)	SOURCE or REFERENCE	COMMENT
6T-028 (203-6)	Ramon Vilella, Barcelona, SPAIN
6T-037 (L128)	Noel L Warner (Becton Dickinson), San Jose, USA
6T-110 (1A4)	Sugita K et al. 1991

SELECTION OF OTHER CD27-SPECIFIC REFERENCE MAB

NAME(Workshop IDs)	SOURCE or REFERENCE	COMMENT
S152	Bigler RD et al. 1988
OKT18	Patricia Rao (Ortho), Raritan, USA
CLB-9F4	van Lier RAW et al. 1987
JML-H235	Mitsuo Yokoyama, Kurume, Japan
O323	Laurence Boumsell, Paris, France
LT27	Filatov AV et al. 1992
143-14	Martorell J et al. 1990
M-T271	E Peter Rieber, Munchen, Germany
VIT14	Walter Knapp, Vienna, Austria

SELECTED REFERENCES ON CD27

REVIEWS

1. Hintzen RQ, de Jong R, Lens SM and van Lier RA. CD27: marker and mediator of T-cell activation? Immunol. Today 1994 15:307 PubMed

PRIMARY CITATIONS

2. Akiba H, et al. CD27, a member of the tumor necrosis factor receptor superfamily, activates NF-kappaB and stress-activated protein kinase/c-Jun N-terminal kinase via TRAF2, TRAF5, and NF-kappaB-inducing kinase. J Biol Chem. 1998 May 22; 273(21): 13353-13358 ** PubMed

3. Agematsu K, Kobata T, Sugita K, Hirose T, Schlossman SF and Morimoto C. Direct cellular communications between CD45R0 and CD45RA T cell subsets via CD27/CD70. J. Immunol. 1995 154:3627 PubMed

4. Bigler RD, Bushkin Y and Chiorazzi N. S152 (CD27). A modulating disulfide-linked T cell activation antigen. J. Immunol. 1988 141:21 PubMed

5. Camerini D, Walz G, Loenen WA, Borst J and Seed B. The T cell activation antigen CD27 is a member of the nerve growth factor/tumor necrosis factor receptor gene family. J. Immunol. 1991 147:3165 PubMed

6. Gravestein LA, van Ewijk W, Ossendorp F and Borst J. CD27 cooperates with the pre-T cell receptor in the regulation of murine T cell development. J. Exp. Med. 1996 184:675 PubMed

7. Jacquot S, Kobata T, Iwata S, Morimoto C and Schlossman SF. CD154/CD40 and CD70/CD27 interactions have different and sequential functions in T cell-dependent B cell responses: enhancement of plasma cell differentiation by CD27 signaling. J. Immunol. 1997 159:2652 PubMed

8. Kobata T, Agematsu K, Kameoka J, Schlossman SF and Morimoto C. CD27 is a signal-transducing molecule involved in CD45RA+ naive T cell costimulation. J. Immunol. 1994 153:5422 PubMed

9. Kobata T, Jacquot S, Kozlowski S, Agematsu K, Schlossman SF and Morimoto C. CD27-CD70 interactions regulate B-cell activation by T cells. Proc. Natl. Acad. Sci. U.S.A. 1995 92:11249 PubMed

10. Loenen WA, De Vries E, Gravestein LA, Hintzen RQ, Van Lier RA and Borst J. The CD27 membrane receptor, a lymphocyte-specific member of the nerve growth factor receptor family, gives rise to a soluble form by protein processing that does not involve receptor endocytosis. Eur. J. Immunol. 1992 22:447 PubMed

11. Prasad KV, Ao Z, Yoon Y, Wu MX, Rizk M, Jacquot S and Schlossman SF. CD27, a member of the tumor necrosis factor receptor family, induces apoptosis and binds to Siva, a proapoptotic protein. Proc. Natl. Acad. Sci. U.S.A. 1997 94:6346 PubMed

12. Sugita K, Torimoto Y, Nojima Y, Daley JF, Schlossman SF and Morimoto C. The 1A4 molecule (CD27) is involved in T cell activation. J. Immunol. 1991 147:1477 PubMed

13. van Lier RA, Borst J, Vroom TM, Klein H, Van Mourik P, Zeijlemaker WP and Melief CJ. Tissue distribution and biochemical and functional properties of Tp55 (CD27), a novel T cell differentiation antigen. J. Immunol. 1987 139:1589 PubMed

WWW RESOURCES

* indicates ammended by reviewer, ** indicates added by reviewer

Portions copyright by Garland Press and by the International Workshops on Human Leukocyte Differentiation Antigens; used with permission

Modified 10/14/99 mpr@mail.nih.gov