PROW: CD82

PROW and IWHLDA present the GUIDE on:
CD82
Author: Helene Conjeaud
Reviewer: Osamu Yoshie
[GUIDE BAR]

ALTERNATE NAMES FOR CD82

4F9
C33
IA4
KAI1
R2

MAJOR LINKS FOR CD82

NCBI LocusLink Record: 3732
Mendelian Inheritance in Man (OMIM): 600623
SwissProt annotated protein record: P27701

FUNCTION BIOCHEMICAL ACTIVITY OF CD82 - No information

CELLULAR FUNCTION OF CD82

Signal transduction
CD82 cross-linking promotes activation of human monocytic cell lines.
Immobilized anti-CD82 induces T cell spreading, pseudopod formation, modulation of T cell proliferation and provides co-stimulatory signals for cytokine production.

DISEASE RELEVANCE OF CD82 AND FUNCTION OF CD82 IN INTACT ANIMAL

CD82 is involved in prostate tumor metastatic potential.
Transfection of human CD82 inhibit the metastatic potential of prostate cancer cells in rat and CD82 expression is correlated with the metastatic potential in human.

STRUCTURE MOLECULAR FAMILY FOR CD82

Families in which CD82 is a member
- CD82-->TM4SF

MOLECULAR STRUCTURE OF CD82

267 amino acids
Four putative transmembrane domains (>23 aa) separating two unequal extracellular loops (27, 119 aa).
The two short ends (9, 15 aa) are located in the cytoplasm.

MOLECULAR MASS OF CD82

CELL TYPE	MW UNREDUCED	MW REDUCED	Comment
Varied cell types	45-90 kDa	Depending on cell type and state of cell activation*

Calculated mw =28kDa peptide core

POST-TRANSCRIPTIONAL MODIFICATION OF CD82 - No information

POST-TRANSLATIONAL MODIFICATION OF CD82

Three N-glycosylation sites in the large extracellular loop. Digestion with N-glycanase reduced the Mr to 28 kDa
No effect of O-glycanase

MOLECULAR INTERACTIONS PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD82 - No information

SUBSTRATES FOR CD82 - No information

ENZYMES WHICH MODIFY CD82 - No information

LIGANDS FOR CD82 AND MOLECULES ASSOCIATED WITH CD82

MOLECULE	COMMENT
Other tetraspan molecules	Ref 7,8,13
MHC-I	Ref 14,15
MHC-II	Ref 7,13,15,
Beta1 integrins	Ref 8,13
CD4	Ref 5,9. The only p56lck-free CD4 is ref9
CD8	Ref 5

EXPRESSION MAIN CELLULAR EXPRESSION OF CD82

Not expressed by red blood cells.
Highly expressed on activated/differentiated hematopoietic cells.
Upregulated in T cells by activation.**

AUTHOR'S ADDITIONAL INSIGHTS ON CD82 - No information

REAGENTS CD82-SPECIFIC MABS NEWLY ASSIGNED AT SIXTH INTERNATIONAL WORKSHOP

NAME(Workshop IDs)	SOURCE or REFERENCE	COMMENT
gC11 (CD82.1	Lebel-Binay S. et al. 1994. Cell. Immunol. 154:468-483
50F11 (CD82.2)	Shaw S and Hallam J NIH, Bethesda

SELECTION OF OTHER CD82-SPECIFIC REFERENCE MAB

NAME(Workshop IDs)	SOURCE or REFERENCE	COMMENT
IA4	Ref 2
4F9	Ref 6
C33	Ref 3

SELECTED REFERENCES ON CD82

REVIEWS

Wright MD,Tomlinson MG The ins and outs of the transmembrane 4 superfamily. Immunol Today 1994 15:588 ** PubMed

Hemler ME,Mannion BA,Berditchevski F Association of TM4SF proteins with integrins: relevance to cancer. Biochim Biophys Acta 1996 1287:67 ** PubMed

PRIMARY CITATIONS

1. Gaugitsch HW,Hofer E,Huber NE,Schnabl E,Baumruker T A new superfamily of lymphoid and melanoma cell proteins with extensive homology to Schistosoma mansoni antigen Sm23. Eur J Immunol 1991 21:377 PubMed

2. Gil ML,Vita N,Lebel-Binay S,Miloux B,Chalon P,Kaghad M,Marchiol-Fournigault C,Conjeaud H,Caput D,Ferrara P,et al A member of the tetra spans transmembrane protein superfamily is recognized by a monoclonal antibody raised against an HLA class I- deficient, lymphokine-activated killer-susceptible, B lymphocyte line. Cloning and preliminary functional studies. J Immunol 1992 148:2826 PubMed

3. Fukudome K,Furuse M,Imai T,Nishimura M,Takagi S,Hinuma Y,Yoshie O Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)- induced syncytium formation: altered glycosylation of C33 antigen in J Virol 1992 66:1394 ** PubMed

4. Imai T,Fukudome K,Takagi S,Nagira M,Furuse M,Fukuhara N,Nishimura M,Hinuma Y,Yoshie O C33 antigen recognized by monoclonal antibodies inhibitory to human T cell leukemia virus type 1-induced syncytium formation is a member of a new family of transmembrane proteins including CD9, CD37, CD53, and CD63. J Immunol 1992 149:2879 ** PubMed

5. Imai T,Yoshie O C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells. J Immunol 1993 151:6470 ** PubMed

6. Nojima Y,Hirose T,Tachibana K,Tanaka T,Shi L,Doshen J,Freeman GJ,Schlossman SF,Morimoto C The 4F9 antigen is a member of the tetra spans transmembrane protein family and functions as an accessory molecule in T cell activation and adhesion. Cell Immunol 1993 152:249 ** PubMed

7. Angelisova P,Hilgert I,Horejsi V Association of four antigens of the tetraspans family (CD37, CD53, TAPA- 1, and R2/C33) with MHC class II glycoproteins. Immunogenetics 1994 39:249 ** PubMed

8. Mannion BA,Berditchevski F,Kraeft SK,Chen LB,Hemler ME Transmembrane-4 superfamily proteins CD81 (TAPA-1), CD82, CD63, and CD53 specifically associated with integrin alpha 4 beta 1 (CD49d/CD29). J Immunol 1996 157:2039 ** PubMed

9. Imai T,Kakizaki M,Nishimura M,Yoshie O Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82. J Immunol 1995 155:1229 PubMed

10. Lebel-Binay S,Lagaudriere C,Fradelizi D,Conjeaud H CD82, tetra-span-transmembrane protein, is a regulated transducing molecule on U937 monocytic cell line. J Leukoc Biol 1995 57:956 PubMed

11. Lebel-Binay S,Lagaudriere C,Fradelizi D,Conjeaud H CD82, member of the tetra-span-transmembrane protein family, is a costimulatory protein for T cell activation. J Immunol 1995 155:101 PubMed

12. Dong JT,Lamb PW,Rinker-Schaeffer CW,Vukanovic J,Ichikawa T,Isaacs JT,Barrett JC KAI1, a metastasis suppressor gene for prostate cancer on human chromosome 11p11.2. Science 1995 268:884 PubMed

13. Rubinstein E,Le Naour F,Lagaudriere-Gesbert C,Billard M,Conjeaud H,Boucheix C CD9, CD63, CD81, and CD82 are components of a surface tetraspan network connected to HLA-DR and VLA integrins. Eur J Immunol 1996 26:2657 PubMed

14. Lagaudriere-Gesbert C,Lebel-Binay S,Wiertz E,Ploegh HL,Fradelizi D,Conjeaud H The tetraspanin protein CD82 associates with both free HLA class I heavy chain and heterodimeric beta 2-microglobulin complexes. J Immunol 1997 158:2790 PubMed

15. Szollosi J,Horejsi V,Bene L,Angelisova P,Damjanovich S Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY. J Immunol 1996 157:2939 PubMed

16. Lebel-Binay S,Gil ML,Lagaudriere C,Miloux B,Marchiol-Fournigault C,Quillet-Mary A,Lopez M,Fradelizi D,Conjeaud H Further characterization of CD82/IA4 antigen (type III surface protein): an activation/differentiation marker of mononuclear cells. Cell Immunol 1994 154:468 PubMed

WWW RESOURCES

* indicates ammended by reviewer, ** indicates added by reviewer

Portions copyright by Garland Press and by the International Workshops on Human Leukocyte Differentiation Antigens; used with permission

Modified 10/14/99 mpr@mail.nih.gov