• lymphocyte function-associated antigen 3, LFA-3

MAJOR LINKS FOR CD58  

• NCBI LocusLink Record: 965
• Mendelian Inheritance in Man (OMIM): 153420
• SwissProt annotated protein record: P19256

CELLULAR FUNCTION OF CD58  

• CD58 mediates adhesion between killer and target cells, antigen presenting cells and T cells, thymocytes and thymic epithelial cells
• Providing costimulatory signal in immune response
• Activation of killer cells

DISEASE RELEVANCE OF CD58 AND FUNCTION OF CD58 IN INTACT ANIMAL   - Unknown

• Families in which CD58 is a member
  • CD58-->CD2/CD48/CD58-->immunoglobulin supergene family

MOLECULAR STRUCTURE OF CD58  

• Extracellular domain (188 amino acids) consists of two immunoglobulin-like domains
• The first N-terminal V domain lacks disulfide bond while the second C2 domain has a disulfide bond
• Anchored to the cellular membrane by two types of mechanisms; transmembrane anchored form and glycosylphosphatidylinositol (GPI)-anchored form

MOLECULAR MASS OF CD58  

POST-TRANSCRIPTIONAL MODIFICATION OF CD58  

• Alternative splicing gives two forms; transmembrane anchored form and GPI-anchored form

POST-TRANSLATIONAL MODIFICATION OF CD58  

• Six potential N-Glycosylation sites are in the extracellular domain

SUBSTRATES FOR CD58   - Unknown

ENZYMES WHICH MODIFY CD58   - Unknown

LIGANDS FOR CD58 AND MOLECULES ASSOCIATED WITH CD58  

• Distributed over many tissues, both hematopoietic and non-hematopoietic cells
• Leukocytes
• Erythrocytes
• Endothelial cells
• Epithelial cells
• Fibroblasts

SELECTION OF OTHER CD58-SPECIFIC REFERENCE MAB  

REVIEWS

1. Springer TA,Dustin ML,Kishimoto TK,Marlin SD The lymphocyte function-associated LFA-1, CD2, and LFA-3 molecules: cell adhesion receptors of the immune system. Annu Rev Immunol 1987 5:223 PubMed

PRIMARY CITATIONS

2. Arulanandam AR,Kister A,McGregor MJ,Wyss DF,Wagner G,Reinherz EL Interaction between human CD2 and CD58 involves the major beta sheet surface of each of their respective adhesion domains. J Exp Med 1994 180:1861 PubMed

3. Dustin ML,Selvaraj P,Mattaliano RJ,Springer TA Anchoring mechanisms for LFA-3 cell adhesion glycoprotein at membrane surface. Nature 1987 329:846 PubMed

4. Osborn L,Day ES,Miller GT,Karpusas M,Tizard R,Meuer SC,Hochman PS Amino acid residues required for binding of lymphocyte function- associated antigen 3 (CD58) to its counter-receptor CD2. J Exp Med 1995 181:429 PubMed

5. Plunkett ML,Sanders ME,Selvaraj P,Dustin ML,Springer TA Rosetting of activated human T lymphocytes with autologous erythrocytes. Definition of the receptor and ligand molecules as CD2 and lymphocyte function-associated antigen 3 (LFA-3). J Exp Med 1987 165:664 PubMed

6. Sanchez-Madrid F,Krensky AM,Ware CF,Robbins E,Strominger JL,Burakoff SJ,Springer TA Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3. Proc Natl Acad Sci U S A 1982 79:7489 PubMed

7. Sanders ME,Makgoba MW,Sharrow SO,Stephany D,Springer TA,Young HA,Shaw S Human memory T lymphocytes express increased levels of three cell adhesion molecules (LFA-3, CD2, and LFA-1) and three other molecules (UCHL1, CDw29, and Pgp-1) and have enhanced IFN-gamma production. J Immunol 1988 140:1401 PubMed

8. Seed B An LFA-3 cDNA encodes a phospholipid-linked membrane protein homologous to its receptor CD2. Nature 1987 329:840 PubMed

9. Semnani RT,Nutman TB,Hochman P,Shaw S,van Seventer GA Costimulation by purified intercellular adhesion molecule 1 and lymphocyte function-associated antigen 3 induces distinct proliferation, cytokine and cell surface antigen profiles in human 'naive' and 'memory' CD4+ T cells. J Exp Med 1994 180:2125 PubMed

10. Wallner BP,Frey AZ,Tizard R,Mattaliano RJ,Hession C,Sanders ME,Dustin ML,Springer TA Primary structure of lymphocyte function-associated antigen 3 (LFA-3). The ligand of the T lymphocyte CD2 glycoprotein. J Exp Med 1987 166:923 PubMed

WWW RESOURCES

Portions copyright by Garland Press and by the International Workshops on Human Leukocyte Differentiation Antigens; used with permission

Modified 10/14/99   mpr@mail.nih.gov